By Arthur E. Humphrey (auth.), Professor Shintaro Furusaki, Dr. Isao Endo, Professor Ryuichi Matsuno (eds.)
Biochemical engineering varieties a bridge among primary biochemical study and massive scale biotechnology tactics. It covers genetic and protein engineering, mobile tradition, bioprocess and reactor layout, separation and modelling. learn paintings in biochemical engineering is an funding sooner or later, while traditional assets should get replaced with renewable ones. during this e-book the papers awarded on the Asia-Pacific Biochemical Engineering convention (Yokohama, Japan 1992) are amassed. This assortment is exclusive in its huge insurance of subject matters and it offers an summary of the present traits of analysis in an incredible area.
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Extra resources for Biochemical Engineering for 2001: Proceedings of Asia-Pacific Biochemical Engineering Conference 1992
Thus we isolated E. coli clones. A plasmid n1:\meg pFeIFNl was recognized to produce FeIFN activity of about 5x10 unit/ml in the third screening. 2 E. 3 Deduced amino acid sequence from cDNA sequence Production in Silkworm The cDNA coding for FeIFN was applied to E. coli, S. cereV2S2ae, CHO, BM-N and in vivo insect larvae, and cultivation condition of each was investigated to increase productivity. 2, we employed recombinant baculovirus and silkworm system for mass production. Characterization of rFeIFN The genetic code of Ser, the third amino acid upstream to an 34 expected N-terminal Cys was changed to Val in order to increase the productivity by preventing one of two possible processing ways and another recombinant baculovirus was prepared for mass production.
Biochem. Eng. (Ed A. Fiechter) 44, 97-121. 13 12. 13. 14. 15. 16. 17. 18. 19. 20. 21. 22. 23. 24. 25. T. L. (1984) Fermentation of lactose by Zymomonas mobilis. J. Biotechnol. 1, 219-228. E. L. (1986) 'Stability of a lac operon in Zymomonas in batch and continuous culture'. J. Biotechnol. 3, 197-205. H. L. (1986) 'Kinetic studies on a lac-containing strain of Zymomonas mobilis'. Biotech. Letts. 8, 807-810. F. L. (1988) 'Cloning and expression of the ~-glucosidase gene from Xanthomonas albilineans in Escherichia coli and Zymomonas ,mobilis'.
A further increase in the enzyme production (60 mg/l) was attained Hhen the fused gene was inserted into a mul ticopy vector, pNU200, and then introduced into B. brevis 47K. The mutant showed higher sensi ti vi ty to various antibiotics than the parental strain, and altered cell Hall and cytoplasmic membrane protein compositions. The results of reversion analysis suggested that a single mutation is responsi ble for these phenotypes and hyperproducti vi ty of human aamylase. As described above, the mutant was also useful for the production of C.