By Marcel Mulder
The sector of artificial membrane technology and know-how is an energetic, transforming into box regarding an interdisciplinary mix of polymer chemistry, actual chemistry, and chemical engineering. a few membrane procedures are quite good understood, and feature been commercialised for a few time period. different membrane procedures have just recently been hired in advertisement purposes, and nonetheless different strategies are just in formative examine phases. This quantity offers a entire compendium of the elemental rules within the box of man-made membranes. the subsequent subject matters are coated: simple points of membrane techniques; fabrics and fabric houses; membrane training and characterisation; membrane delivery; focus polarisation and fouling; approach and module layout. the second one variation of this well-established textual content has been significantly multiplied and up to date, and plenty of chapters now comprise sections giving solved and unsolved difficulties. viewers: This ebook is suggested as a textbook for undergraduate and graduate scholars, in addition to a finished reference for engineers, scientists, and technical administration.
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Additional info for Basic Principles of Membrane Technology, Second Edition
The utility of the planar lipid bilayer technique resides in the opportunity it provides to study channel activity in biophysical terms in the absence of a complicating and sometimes confounding cell 1. Structure/Function in abENaC 21 system. Although the subtleties of channel gating, amiloride affinity, and ion selectivity may be hidden when investigating macroscopic cell function, the study of these parameters allows us to begin to focus on the individual amino acid residues that regulate channel and ultimately cellular behavior.
The current/voltage relations for wt abENaC and K504E and K515E abENaC are shown on the top panel. Neither mutation affected single-channel conductance recorded under symmetrical conditions. The ion selectivity of wild-type and mutant ENaC subunits was determined under bi-ionic conditions, with 100 mM Na+ in the trans compartment and 100 mM K+ in the cis chamber, and is shown in the bottom panel. Values are mean t SD for 4 to 7 experiments at each voltage, as calculated from events histogram analysis.
Sorscher, E. , Brinkley, B. , and Benos, D. J. (1989). Immunochemical localization of amiloride-sensitive sodium channels in sodium transporting epithelia. J. Cell Sci. 93, 349-362. , and Lazdunski, M. (1995a). Functional degenerin-containing chimeras identify residues essential for arniloride-sensitive Na+ channel function. J. Biol. Chem. 270,11735-1 1737. , and Lazdunski, M. (1995b). Molecular cloning and functional expression of a novel amiloride-sensitive Na’ channel. J. B i d . Chem. 270,27411-27414.